Overview

  • Product nameAnti-Syntenin antibody
    See all Syntenin primary antibodies
  • Description
    Rabbit polyclonal to Syntenin
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to internal sequence amino acids 109-158 of Human Syntenin (KQGIREVILC KDQDGKIGLR LKSIDNGIFV QLVQANSPAS LVGLRFGDQV)

  • Positive control
    • HepG2 cell lysate. Human kidney tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionSeems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.
  • Tissue specificityWidely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
  • Sequence similaritiesContains 2 PDZ (DHR) domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues.
  • Cellular localizationCell junction > focal adhesion. Cell junction > adherens junction. Cell membrane. Endoplasmic reticulum membrane. Nucleus. Melanosome. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • MDA-9 antibody
    • MDA9 antibody
    • Melanoma differentiation-associated protein 9 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 18 antibody
    • Scaffold protein Pbp1 antibody
    • SDCB1_HUMAN antibody
    • SDCBP antibody
    • ST1 antibody
    • SYCL antibody
    • Syndecan binding protein (syntenin) antibody
    • Syndecan binding protein 1 antibody
    • Syndecan-binding protein 1 antibody
    • Syntenin 1 antibody
    • Syntenin-1 antibody
    • TACIP18 antibody
    see all

Anti-Syntenin antibody images

  • All lanes : Anti-Syntenin antibody (ab62530) at 1.25 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Syntenin with ab62530 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-Syntenin antibody (ab62530)

ab62530 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62530.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"