Overview

  • Product nameAnti-Syntrophin alpha 1 antibody
    See all Syntrophin alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Syntrophin alpha 1
  • SpecificityThis antibody recognizes alpha 1 syntrophin (60 kDa) but does not react with beta 1 and beta 2 syntrophins.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 191-206 of Mouse Syntrophin alpha 1.

  • General notesFor continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/1000.
ICC/IF 1/200.
WB 1/2000. Predicted molecular weight: 60 kDa.

Target

  • FunctionAdapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate.
  • Tissue specificityHigh expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
  • Involvement in diseaseDefects in SNTA1 are the cause of long QT syndrome type 12 (LQT12) [MIM:612955]. A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
  • Sequence similaritiesBelongs to the syntrophin family.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 SU (syntrophin unique) domain.
  • DomainThe PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-biphosphate.
    The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
    The SU domain binds calmodulin in a calcium-dependent manner.
  • Post-translational
    modifications
    Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
  • Cellular localizationCell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
    • Acidic alpha 1 syntrophin antibody
    • Alpha 1 syntrophin antibody
    • Alpha-1-syntrophin antibody
    • dJ1187J4.5 antibody
    • Dystrophin associated protein A1 59kDa acidic component antibody
    • LQT12 antibody
    • OTTHUMP00000030650 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody
    • SNT1 antibody
    • Snta1 antibody
    • SNTA1_HUMAN antibody
    • Syntrophin 1 antibody
    • Syntrophin-1 antibody
    • TACIP1 antibody
    see all

References for Anti-Syntrophin alpha 1 antibody (ab11187)

This product has been referenced in:
  • Anderova M  et al. Altered astrocytic swelling in the cortex of a-syntrophin-negative GFAP/EGFP mice. PLoS One 9:e113444 (2014). WB ; Mouse . Read more (PubMed: 25426721) »
  • Koo T  et al. Delivery of AAV2/9-Microdystrophin Genes Incorporating Helix 1 of the Coiled-Coil Motif in the C-Terminal Domain of Dystrophin Improves Muscle Pathology and Restores the Level of a1-Syntrophin and a-Dystrobrevin in Skeletal Muscles of mdx Mice. Hum Gene Ther : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21453126) »

See all 4 Publications for this product

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I am very sorry that this product did not work as expected and wish to apologize for the inconvenience this has caused. In reviewing the protocol details which you have provided I see no reason why t...

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I am very sorry to hear that you have been having difficulties when using this recent lot in ICC/IF. Sadly, the lot which you have requested, 79335, is not longer in stock. However, I would be very happy to try...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (rat spinal cord cytosolic and membranous extract)
Loading amount 40 µg
Specification rat spinal cord cytosolic and membranous extract
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (skeletal muscle)
Specification skeletal muscle
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%
Username

Dr. bruno Constantin

Verified customer

Submitted Mar 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"