Anti-CREST antibody (ab126126)
Key features and details
- Rabbit polyclonal to CREST
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CREST antibody
See all CREST primary antibodies -
Description
Rabbit polyclonal to CREST -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment within Human CREST aa 200-383. The exact sequence is proprietary.
Database link: O75177 -
Positive control
- WB: MCF7 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab126126 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
Target
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Function
Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. -
Tissue specificity
Ubiquitous; with lowest levels in spleen. -
Sequence similarities
Belongs to the SS18 family. -
Domain
The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to nuclear bodies. Co-localizes with SGOL1 at kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 26039 Human
- Entrez Gene: 269397 Mouse
- Entrez Gene: 192352 Rat
- Omim: 606472 Human
- SwissProt: O75177 Human
- SwissProt: Q8BW22 Mouse
- SwissProt: Q91XJ0 Rat
- Unigene: 154429 Human
see all -
Alternative names
- Calcium-responsive transactivator antibody
- CREST antibody
- CREST_HUMAN antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126126 has not yet been referenced specifically in any publications.