Anti-SYT2 antibody (ab60716)

Overview

  • Product nameAnti-SYT2 antibody
    See all SYT2 primary antibodies
  • Description
    Mouse monoclonal to SYT2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rhesus monkey, Opossum
  • Immunogen

    Recombinant fragment with tag: KIHLMQNGKR LKKKKTTVKK KTLNPYFNES FSFEIPFEQI QKVQVVVTVL DYDKLGKNEA IGKIFVGSNA TGTELRHWSD MLANPRRPIA QWHSLKPEEE VDALLGKN, corresponding to amino acids 311-419 of Human SYT2

  • Positive control
    • HepG2 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab60716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution. The detection limit for recombinant tagged SYT2 is approximately 0.1ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
    • Sequence similaritiesBelongs to the synaptotagmin family.
      Contains 2 C2 domains.
    • DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
      The second C2 domain mediates interaction with Stonin 2.
    • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Synaptotagmin 2 antibody
      • Synaptotagmin II antibody
      • Synaptotagmin-2 antibody
      • Synaptotagmin2 antibody
      • SynaptotagminII antibody
      • SYT 2 antibody
      • Syt II antibody
      • Syt2 antibody
      • SYT2_HUMAN antibody
      • SytII antibody
      see all

    Anti-SYT2 antibody images

    • Anti-SYT2 antibody (ab60716) at 1 µg/ml + HepG2 cell lysate at 25 µg

      Predicted band size : 47 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-SYT2 antibody (ab60716)

    ab60716 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein (partial recombinant protein)
    Loading amount 0.02 µg
    Specification partial recombinant protein
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mr. Benjamin Wilhelm

    Verified customer

    Submitted Apr 18 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - other (Brain)
    Loading amount 10 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 18 2012

    DISCOUNT CODE: x

    Ablaufdatum: ddmm2012
    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab60716 in Maus, Ratte und Humanem Gewebe zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf eines w...

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    Vielen Dank für Ihre Rückmeldung.
    Die Auswahl des Immunogens basiert oftmals auf Vorhersagen, wo in einem Protein sich geeignete Immunogenpeptidsequenzen befinden können. Oder es wird der Proteinteil zugrunde gelegt, gegen den ein Antikörper produ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"