Overview

  • Product name
    Anti-SYT2 antibody [ZNP-1]
    See all SYT2 primary antibodies
  • Description
    Mouse monoclonal [ZNP-1] to SYT2
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Does not react with: Cow
  • Immunogen

    Zebrafish Organism.

  • Positive control
    • Human, Rat, Mouse and Zebrafish Brain Homogenate Lysate; SH-SY5Y cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: Human serum albumin
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Near homogeneity as judged by SDS-PAGE. ab154035 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.
  • Clonality
    Monoclonal
  • Clone number
    ZNP-1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
  • Sequence similarities
    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • Domain
    The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with Stonin 2.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Synaptotagmin 2 antibody
    • Synaptotagmin II antibody
    • Synaptotagmin-2 antibody
    • Synaptotagmin2 antibody
    • SynaptotagminII antibody
    • SYT 2 antibody
    • Syt II antibody
    • Syt2 antibody
    • SYT2_HUMAN antibody
    • SytII antibody
    see all

Images

  • All lanes : Anti-SYT2 antibody [ZNP-1] (ab154035) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Brain Homogenate Lysate
    Lane 3 : Mouse Brain Homogenate Lysate
    Lane 4 : Zebrafish Brain Homogenate Lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG – HRP at 1/1000 dilution

    Predicted band size : 48 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry with SH-SY5Y cells stained with Anti-SYT2 antibody (ab154035) in red and DAPI in blue, as a counter stain. The target protein locates to the plasma membrane (red).

    Fixation: 4% Paraformaldehyde, 20 min.
    Permeabilization: 0.1% Triton X-100, 10min.
    Blocking: 10% Goat Serum/PBS, 1 hour.
    Primary: Anti-SYT2 antibody (ab154035) 5 µg/ml in 10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C.
    Secondary: Alexa Fluor® 594 Goat Anti-Mouse (H&L) used at a 1/1000 dilution in 10% Goat Serum/PBS, 1hr at RT.
    Washing: 3x 1% Goat Serum/PBS, 10 mins/wash
    DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins

References

ab154035 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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