Overview

  • Product name
  • Description
    Rabbit polyclonal to SYVN1
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, from the C-terminal region of Human SYVN1 (NP_115807.1).

  • Positive control
    • Human kidney and liver tissues; T47D cell and Mouse kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab118483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 67 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.

Target

  • Function
    Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the HRD1 family.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger is required for E3 ligase activity.
  • Post-translational
    modifications
    Not N-glycosylated.
    Auto-ubiquitinated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200010C09Rik antibody
    • DER3 antibody
    • E3 ubiquitin-protein ligase synoviolin antibody
    • HMG coA reductase degradation 1 homolog antibody
    • HRD1 antibody
    • KIAA1810 antibody
    • MGC40372 antibody
    • OTTHUMP00000230429 antibody
    • OTTHUMP00000230430 antibody
    • OTTHUMP00000230431 antibody
    • OTTHUMP00000230432 antibody
    • Synovial apoptosis inhibitor 1 antibody
    • Synovial apoptosis inhibitor 1, synoviolin antibody
    • Synoviolin 1 antibody
    • Synoviolin 1 isoform b antibody
    • SYNOVIOLIN antibody
    • SYVN1 antibody
    • SYVN1_HUMAN antibody
    see all

Images

  • ab118483 at 10µg/ml staining SYVN1 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab118483 at 10µg/ml staining SYVN1 in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-SYVN1 antibody (ab118483) at 1/100 dilution + T47D cell lysate at 35 µg

    Predicted band size : 67 kDa
  • Anti-SYVN1 antibody (ab118483) at 1/100 dilution + Mouse kidney tissue lysate

    Predicted band size : 67 kDa

References

ab118483 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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