Anti-SYVN1 antibody - Carboxyterminal end (ab38456)


  • Product name
    Anti-SYVN1 antibody - Carboxyterminal end
    See all SYVN1 primary antibodies
  • Description
    Rabbit polyclonal to SYVN1 - Carboxyterminal end
  • Specificity
    This antibody is specific for SYVN1.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected within aa 600-650 of human SYVN1.

  • Positive control
    • Breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab38456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 68 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the HRD1 family.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger is required for E3 ligase activity.
  • Post-translational
    Not N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200010C09Rik antibody
    • DER3 antibody
    • E3 ubiquitin-protein ligase synoviolin antibody
    • HMG coA reductase degradation 1 homolog antibody
    • HRD1 antibody
    • KIAA1810 antibody
    • MGC40372 antibody
    • OTTHUMP00000230429 antibody
    • OTTHUMP00000230430 antibody
    • OTTHUMP00000230431 antibody
    • OTTHUMP00000230432 antibody
    • Synovial apoptosis inhibitor 1 antibody
    • Synovial apoptosis inhibitor 1, synoviolin antibody
    • Synoviolin 1 antibody
    • Synoviolin 1 isoform b antibody
    • SYNOVIOLIN antibody
    • SYVN1 antibody
    • SYVN1_HUMAN antibody
    see all


  • Anti-SYVN1 antibody - Carboxyterminal end (ab38456) at 1/100 dilution + mouse kidney tissue lysate

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38456 at a 1/50 dilution.
  • ICC/IF image of ab38456 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38456, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Bianchini E  et al. Unveiling the degradative route of the V247M a-sarcoglycan mutant responsible for LGMD-2D. Hum Mol Genet N/A:N/A (2014). WB ; Human . Read more (PubMed: 24565866) »
  • Yamada T  et al. WFS1-deficiency increases endoplasmic reticulum stress, impairs cell cycle progression and triggers the apoptotic pathway specifically in pancreatic beta-cells. Hum Mol Genet 15:1600-9 (2006). Read more (PubMed: 16571599) »

See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Human Cell lysate - whole cell (NSCLC)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jun 25 2013


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