Overview

  • Product name
    Anti-SYVN1 antibody [EP7459]
    See all SYVN1 primary antibodies
  • Description
    Rabbit monoclonal [EP7459] to SYVN1
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SYVN1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q86TM6

  • Positive control
    • 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab170901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
    • Tissue specificity
      Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the HRD1 family.
      Contains 1 RING-type zinc finger.
    • Domain
      The RING-type zinc finger is required for E3 ligase activity.
    • Post-translational
      modifications
      Not N-glycosylated.
      Auto-ubiquitinated.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1200010C09Rik antibody
      • DER3 antibody
      • E3 ubiquitin-protein ligase synoviolin antibody
      • HMG coA reductase degradation 1 homolog antibody
      • HRD1 antibody
      • KIAA1810 antibody
      • MGC40372 antibody
      • OTTHUMP00000230429 antibody
      • OTTHUMP00000230430 antibody
      • OTTHUMP00000230431 antibody
      • OTTHUMP00000230432 antibody
      • Synovial apoptosis inhibitor 1 antibody
      • Synovial apoptosis inhibitor 1, synoviolin antibody
      • Synoviolin 1 antibody
      • Synoviolin 1 isoform b antibody
      • SYNOVIOLIN antibody
      • SYVN1 antibody
      • SYVN1_HUMAN antibody
      see all

    Images

    • Immunofluorescence analysis of HeLa cells labeling SYVN1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).

    • Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1 using ab170901 at a 1/10 dilution

    • All lanes : Anti-SYVN1 antibody [EP7459] (ab170901) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : HepG2 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labeled goat anti-rabbit. at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 67 kDa
    • Flow cytometry analysis of permeabilized 293T cells labeling SYVN1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).

    References

    This product has been referenced in:
    • Feng L  et al. Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant/a-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy. Autophagy 13:686-702 (2017). Read more (PubMed: 28121484) »
    • Kong S  et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A 113:10394-9 (2016). Read more (PubMed: 27573825) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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