Overview

  • Product nameAnti-T-bet / Tbx21 antibody
    See all T-bet / Tbx21 primary antibodies
  • Description
    Rabbit polyclonal to T-bet / Tbx21
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human T-bet/ Tbx21.

    (Peptide available as ab69655.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, Jurkat Nuclear, Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM), E14Tg2a Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab53174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).

Target

  • FunctionTranscription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
  • Tissue specificityT-cell specific.
  • Involvement in diseaseGenetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
  • Sequence similaritiesContains 1 T-box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • T bet antibody
    • T box 21 antibody
    • T box expressed in T cells antibody
    • T box protein 21 antibody
    • T box transcription factor TBX21 antibody
    • T cell specific T box transcription factor antibody
    • T cell specific T box transcription factor T bet antibody
    • T PET antibody
    • T-box protein 21 antibody
    • T-box transcription factor TBX21 antibody
    • T-cell-specific T-box transcription factor T-bet antibody
    • TBET antibody
    • TBLYM antibody
    • TBX 21 antibody
    • Tbx21 antibody
    • TBX21_HUMAN antibody
    • TPET antibody
    • Transcription factor TBLYM antibody
    see all

Anti-T-bet / Tbx21 antibody images

  • T-bet / Tbx21 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53174.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 53kDa; T-bet / Tbx21

  • All lanes : Anti-T-bet / Tbx21 antibody (ab53174) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-T-bet / Tbx21 antibody (ab53174)

ab53174 has not yet been referenced specifically in any publications.

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