Anti-T Cell Factor 3 + 4 antibody [6F12-3] - ChIP Grade (ab12065)

Overview

  • Product nameAnti-T Cell Factor 3 + 4 antibody [6F12-3] - ChIP Grade
  • Description
    Mouse monoclonal [6F12-3] to T Cell Factor 3 + 4 - ChIP Grade
  • Tested applicationsSuitable for: WB, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with a His-tagged fusion protein corresponding to amino acids 31-331 of Human TCF4.

  • Positive control
    • Cos1 cells transfected with human TCF3.

Properties

Applications

Our Abpromise guarantee covers the use of ab12065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA
WB
IHC-P
ChIP
  • Application notesChIP: Use at an assay dependent dilution(PMID 20159597)
    GSA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 60 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceT-cell factor 3 and 4 (TCF3 and TCF4) are transcription factors of the High Mobility Group of DNA binding proteins. They are members of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. TCF4 generates transcriptionally active complexes with beta catenin in colon carcinoma cells. TCF3 appears to be expressed independently of TCF4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • ITF1 antibody
      • ITF2 antibody
      • TCF3 antibody
      • TCF4 antibody
      • Transcription factor 3 antibody
      • Transcription factor 4 antibody
      • XTCF3 antibody
      see all

    References for Anti-T Cell Factor 3 + 4 antibody [6F12-3] - ChIP Grade (ab12065)

    This product has been referenced in:
    • Tian Y  et al. Characterization and In Vivo Pharmacological Rescue of a Wnt2-Gata6 Pathway Required for Cardiac Inflow Tract Development. Dev Cell 18:275-287 (2010). ChIP ; Mouse . Read more (PubMed: 20159597) »
    • Balaz P  et al. TCF-3, 4 protein expression correlates with beta-catenin expression in MSS and MSI-H colorectal cancer from HNPCC patients but not in sporadic colorectal cancers. Int J Colorectal Dis 25:931-9 (2010). IHC-P ; Human . Read more (PubMed: 20532534) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry regarding ab12065. All the information we have for this antibody is made available to you on our online datasheet at www.Abcam.com. Due to the size of our catalog we have unfortunately not tested this antibody ourselves. Howe...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"