The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceTwo distinct types of T-cell antigen receptors have been identified: the alpha/beta heterodimer found on functional helper and cytotoxic T cells, and the gamma/delta heterodimer. The latter is first detected approximately 2 days before the appearance of cell-surface alpha/beta heterodimer during T-cell ontogeny.
ab114063 at 1µg/106 cells staining T Cell Receptor in Mouse Balb/c TCR Positive Thymocytes by Flow Cytometry.
Percentage of cells stained above control (Purified Mouse IgG2a): 96.7%.
References for Anti-T Cell Receptor antibody [KJ1-26] (ab114063)
This product has been referenced in:
Marrack P et al. The major histocompatibility complex-restricted antigen receptor on T cells. IV. An antiidiotypic antibody predicts both antigen and I-specificity. J Exp Med158:1635-46 (1983).
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