The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceTwo distinct types of T-cell antigen receptors have been identified: the alpha/beta heterodimer found on functional helper and cytotoxic T cells, and the gamma/delta heterodimer. The latter is first detected approximately 2 days before the appearance of cell-surface alpha/beta heterodimer during T-cell ontogeny.