Anti-T Cell Receptor V gamma 3 antibody [536] (ab21029)

Overview

  • Product nameAnti-T Cell Receptor V gamma 3 antibody [536]
    See all T Cell Receptor V gamma 3 primary antibodies
  • Description
    Syrian Hamster monoclonal [536] to T Cell Receptor V gamma 3
  • Specificityab21029 stains <1% of cells in adult thymus (AKR), splenocytes, lymph node, adult CD4-8- thymocytes and adult CD4-8-, T splenocyte. 92% of day 14 old Balb/c fetal thymus, 86% of C57BL/6 dEC (Thy-1+) and 94% of AKR dEC (Thy-1+) stain positive using this antibody.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • EpitopeThis antibody recognizes an epitope of the variable region (V gamma 3) gene product.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number536
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC/IF
IP
  • Application notesFlow Cyt: Use 1µg for 106 cells.
    IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution. ab21029 immunoprecipitates the TCR heterdimer with two subunits of apparent molecular mass of 52 and 42kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceT cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable) , J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the gamma locus are known to be incapable of encoding a protein and are considered pseudogenes. Somatic rearrangment of the gamma locus has been observed in T cells derived from patients with T cell leukemia and ataxia telangiectasia.
    • Cellular localizationMembrane; Single-pass membrane protein
    • Database links
    • Alternative names
      • T cell rearranging gene gamma antibody
      • T cell receptor gamma locus antibody
      • T-cell receptor gamma chain C region PT-gamma-1/2 antibody
      • T-cell receptor gamma-2 chain C region antibody
      • tcrg antibody
      • TCRGC2 antibody
      • trg antibody
      • TRGC2 antibody
      • TRGC2(2X) antibody
      • TRGC2(3X) antibody
      see all

    References for Anti-T Cell Receptor V gamma 3 antibody [536] (ab21029)

    ab21029 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam.

    I am unable to locate the rat sequence for T Cell Receptor V gamma 3, without this sequence, I am unable to say one way or the other, whether ab21209 would work on rat tissue or not. If you could please provide ...

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    Thank you for contacting Abcam and for your interest in our products.

    Regarding the CD4 antibody ab25804, based on a BLAST of the immunogen, there is no homology with rat, therefore I would not recommend this antibody to use with rat samples...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"