Overview

  • Product name
  • Description
    Rabbit polyclonal to T7 tag®
  • Specificity
    ELISA: The anti T7 diluted 1:176,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Immunogen

    MASMTGGQQMG (T7 epitope) conjugated to KLH.

  • General notes
    T7-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Relevance
    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

References

This product has been referenced in:
  • Ando M  et al. The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity. Mol Neurodegener 12:32 (2017). Read more (PubMed: 28438176) »
  • Horn AV  et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 24101588) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Ab9115 and ab18184 are provided at 1mg/ml. For both antibodies I would recommend an initial dilution 1/1000 and try serial dilutions in order to optimise it to your experiment.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Jan 27 2006

Thank you very much for those details, it is very useful. I am sorry you are not satisfied with ab9115. In this instance, without admission of responsibility or liability, I am prepared to offer you a credit note or refund for the value of the antib...

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This antibody has not yet been tested in ICC. We have two further antibodies to T7 epitope tag (ab9112 an ab9138), but neither of these have been tested in ICC !

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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