Overview

  • Product name
    Anti-T7 tag® antibody (Agarose)
    See all T7 tag® primary antibodies
  • Description
    Rabbit polyclonal to T7 tag® (Agarose)
  • Conjugation
    Agarose
  • Tested applications
    Suitable for: IPmore details
  • Species reactivity
    Supplier confirmed has not performed species reactivity for the product.
  • Immunogen

    T7 tag (MASMTGGQQMG) conjugated to KLH.

  • General notes
    Amount: 0.1 mg antibody/200 µl of gel

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.1M Sodium chloride, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab90665 was affinity purified using the peptide immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use 15-25 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
    • Alternative names
      • 10 antibody
      • Major capsid protein 10A antibody
      • Major capsid protein antibody
      • Minor capsid protein 10B antibody
      • T7 epitope tag antibody
      see all

    References

    ab90665 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab90665.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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