The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use 15-25 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
Major capsid protein 10A antibody
Major capsid protein antibody
Minor capsid protein 10B antibody
T7 epitope tag antibody
References for Anti-T7 tag® antibody (Agarose) (ab90665)
has not yet been referenced specifically in any publications.
Publishing research using ab90665? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"