Overview

  • Product name
    Anti-T7 tag® antibody (HRP)
    See all T7 tag® primary antibodies
  • Description
    Rabbit polyclonal to T7 tag® (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISA, ICCmore details
  • Immunogen

    Synthetic peptide:

    MASMTGGQQMG

    conjugated to KLH.

  • General notes
    T7-TAG® is a trademark of EMD Biosciences, Inc.

    Molar enzyme/antibody protein ratio is 4:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES. pH 7.1
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC
  • Application notes
    ELISA: 1/10000 - 1/100000.
    ICC: 1/200 - 1/500.
    WB: 1/1000 - 1/30000. Colorimetric detection: 1:1000 - 1:10000, Chemiluminescent detection: 1:1000 - 1:30000

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
    • Alternative names
      • 10 antibody
      • Major capsid protein 10A antibody
      • Major capsid protein antibody
      • Minor capsid protein 10B antibody
      • T7 epitope tag antibody
      see all

    References for Anti-T7 tag® antibody (HRP) (ab19291)

    ab19291 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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