Overview

  • Product name
    Anti-T7 tag® antibody (HRP)
    See all T7 tag® primary antibodies
  • Description
    Rabbit polyclonal to T7 tag® (HRP)
  • Conjugation
    HRP
  • Specificity
    Ab21477 recognises T7 tag.
  • Tested applications
    Suitable for: ELISA, WB, ICCmore details
  • Species reactivity
    May react with Bordetella phage BPP-1, Yersinia pestis phage phiA1122, Bacteriophage T7, based on sequence homology. Not yet tested in other species.
  • Immunogen

    Synthetic peptide:

    MASMTGGQQMG

    conjugated to KLH, corresponding to amino acids 1-11 of T7 tag

  • General notes
    T7-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 50mM HEPES, 100mM Sodium chloride, pH 7.1
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance
    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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