Anti-T7 tag® antibody (Phycoerythrin) (ab72563)

Overview

  • Product nameAnti-T7 tag® antibody (Phycoerythrin)
    See all T7 tag® primary antibodies
  • Description
    Rabbit polyclonal to T7 tag® (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Immunogen

    T7 epitope tag conjugated to a carrier protein.

  • General notes

    Excitation maximum: 565>498 nm Emission maximum: 578 nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PuritySize exclusion
  • Purification notesSize exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab37409-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Anti-T7 tag® antibody (Phycoerythrin) images

  • Flow cytometry analysis of a recombinant (N-terminus T7-tag®) transmembrane cell surface receptor in CHO cells using ab72563 (yellow). Black - Mouse IgG1 Phycoerythrin isotype control.

  • Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated T7-tagged peptide and read after a 2 hour incubation at room temperature with ab72563.

References for Anti-T7 tag® antibody (Phycoerythrin) (ab72563)

ab72563 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"