Anti-T7 tag® antibody (SureLight® Allophycocyanin) (ab72565)

Overview

  • Product name
    Anti-T7 tag® antibody (SureLight® Allophycocyanin)
    See all T7 tag® primary antibodies
  • Description
    Rabbit polyclonal to T7 tag® (SureLight® Allophycocyanin)
  • Conjugation
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Synthetic peptide T7 tag conjugated to a carrier molecule

  • General notes

    Excitation maximum:650 nm Emission maximum:660 nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Size exclusion chromatography as well as some proprietary methods for purifying the final materials werer used.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.

Target

  • Relevance
    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Images

  • Flow cytometry analysis of a recombinant (N-terminus T7-tag) transmembrane cell surface receptor in CHO cells using ab72565 at a 1/20 dilution (yellow). Black - Mouse IgG SureLight® Allophycocyanin isotype control.

References

This product has been referenced in:
  • de la Fuente M  et al. Mapping human protease-activated receptor 4 (PAR4) homodimer interface to transmembrane helix 4. J Biol Chem 287:10414-23 (2012). Flow Cyt . Read more (PubMed: 22318735) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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