Anti-T7 tag® antibody [T7 Tag] (ab50545)

Overview

  • Product nameAnti-T7 tag® antibody [T7 Tag]
    See all T7 tag® primary antibodies
  • Description
    Mouse monoclonal [T7 Tag] to T7 tag®
  • Tested applicationsSuitable for: WBmore details
  • Immunogen

    Synthetic peptide:

    MASMTGGQQMG-K

    , corresponding to amino acids 1-11 of Bacteriophage T7 gene10 (T7 tag®) conjugated to KLH.

  • Positive control
    • Recombinant T7 tagged beta galactosidase.
  • General notesT7-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purification notesAb50545 is from a purified immunoglobulin.
  • ClonalityMonoclonal
  • Clone numberT7 Tag
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml.

Target

  • RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

References for Anti-T7 tag® antibody [T7 Tag] (ab50545)

ab50545 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Dictyostelium discoideum Cell lysate - whole cell (single cells)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification single cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 07 2015

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab9138.

To check the status of the order please contact our...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Fusion protein)
Loading amount 25 µg
Specification Fusion protein
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"