Anti-TAB1 antibody (ab151408)
Key features and details
- Rabbit polyclonal to TAB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-TAB1 antibody
See all TAB1 primary antibodies -
Description
Rabbit polyclonal to TAB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 257 of Human TAB1 (Uniprot ID: Q15750)
-
Positive control
- GL261, C8D30, 293T, A431 and A375 whole cell lysate/extracts. HeLa cells. Human colon cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 PP2C-like domain. -
Post-translational
modificationsMonoubiquitinated. Deubiquitinated by Y.enterocolitica YopP. - Information by UniProt
-
Database links
- Entrez Gene: 10454 Human
- Entrez Gene: 66513 Mouse
- Omim: 602615 Human
- SwissProt: Q15750 Human
- SwissProt: Q8CF89 Mouse
- Unigene: 507681 Human
- Unigene: 288245 Mouse
-
Alternative names
- 2310012M03Rik antibody
- 3'-Tab1 antibody
- MAP3K7IP 1 antibody
see all
Images
-
All lanes : Anti-TAB1 antibody (ab151408) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TAB1 knockout HEK-293T cell lysate
Lane 3 : U-2 OS cell lysate
Lane 4 : HeLa cell lysate
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-TAB1 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151408 was shown to bind specifically to TAB1. A band was observed at 60 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TAB1 knockout cell line ab266845 (knockout cell lysate ab258220). To generate this image, wild-type and TAB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 �C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
-
All lanes : Anti-TAB1 antibody (ab151408) at 1/1000 dilution
Lane 1 : GL261 whole cell lysate/extract
Lane 2 : C8D30 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS-PAGE -
All lanes : Anti-TAB1 antibody (ab151408) at 1/1000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : A375 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS-PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TAB1 with ab151408 at 1/100 dilution.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling TAB1 with ab151408 at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab151408 has been referenced in 2 publications.
- Rodrigues M et al. TAK1 protein kinase activity is required for TLR signalling and cytokine production in myeloid cells. Biochem J 479:1891-1907 (2022). PubMed: 36062803
- Zhang J et al. Interleukin-1 and TRAF6-dependent activation of TAK1 in the absence of TAB2 and TAB3. Biochem J 474:2235-2248 (2017). PubMed: 28507161