Overview

  • Product nameAnti-TAB1 antibody
    See all TAB1 primary antibodies
  • Description
    Rabbit polyclonal to TAB1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide mapping to the C terminus of Human TAB1.

  • Positive control
    • BaF3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab27983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).

Target

  • FunctionMay be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 PP2C-like domain.
  • Post-translational
    modifications
    Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310012M03Rik antibody
    • 3'-Tab1 antibody
    • MAP3K7IP 1 antibody
    • MAP3K7IP1 antibody
    • MGC57664 antibody
    • Mitogen activated protein kinase kinase kinase 7 interacting protein 1 antibody
    • Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 antibody
    • TAB 1 antibody
    • TAB1 antibody
    • TAB1_HUMAN antibody
    • TAK1 binding protein 1 antibody
    • TAK1-binding protein 1 antibody
    • TGF beta activated kinase 1 binding protein 1 antibody
    • TGF-beta activated kinase 1/MAP3K7 binding protein 1 antibody
    • TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 antibody
    • TGF-beta-activated kinase 1-binding protein 1 antibody
    • Transforming growth factor beta activated kinase binding protein 1 antibody
    see all

Anti-TAB1 antibody images

  • All lanes : Anti-TAB1 antibody (ab27983) at 1/1000 dilution

    Lane 1 : CCL13 whole cell lysate, untransfected
    Lane 2 : CCL13 whole cell lysate, TAB1 transfected

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alexa Fluor® 680 conjugated goat anti-rabbit antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Miss Nicola Bright

    See Abreview

References for Anti-TAB1 antibody (ab27983)

ab27983 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization Yes - 0.2% triton x100
Specification Brain
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 14 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (CCL13)
Loading amount 30 µg
Specification CCL13
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Miss. Nicola Bright

Verified customer

Submitted Jun 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"