Overview

  • Product name
    Anti-TAB1 antibody [EPR635Y]
    See all TAB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR635Y] to TAB1
  • Tested applications
    Suitable for: IHC-P, WB, IP, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human TAB1.

  • Positive control
    • MCF-7 cell lysate; human tonsil tisssue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Predicted molecular weight: 55 kDa.
IP 1/30.
ICC 1/50 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 PP2C-like domain.
  • Post-translational
    modifications
    Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310012M03Rik antibody
    • 3'-Tab1 antibody
    • MAP3K7IP 1 antibody
    • MAP3K7IP1 antibody
    • MGC57664 antibody
    • Mitogen activated protein kinase kinase kinase 7 interacting protein 1 antibody
    • Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 antibody
    • TAB 1 antibody
    • TAB1 antibody
    • TAB1_HUMAN antibody
    • TAK1 binding protein 1 antibody
    • TAK1-binding protein 1 antibody
    • TGF beta activated kinase 1 binding protein 1 antibody
    • TGF-beta activated kinase 1/MAP3K7 binding protein 1 antibody
    • TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 antibody
    • TGF-beta-activated kinase 1-binding protein 1 antibody
    • Transforming growth factor beta activated kinase binding protein 1 antibody
    see all

Anti-TAB1 antibody [EPR635Y] images

  • Overlay histogram showing HeLa cells stained with ab76412 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76412, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-TAB1 antibody [EPR635Y] (ab76412) at 1/2000 dilution + MCF-7 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunohistochemical staining of TAB1 in paraffin embedded human tonsils using ab76412 at a 1/100 dilution.

References for Anti-TAB1 antibody [EPR635Y] (ab76412)

This product has been referenced in:
  • Huang HL  et al. Targeting of TGF-ß-activated protein kinase 1 inhibits chemokine (C-C motif) receptor 7 expression, tumor growth and metastasis in breast cancer. Oncotarget 6:995-1007 (2015). Read more (PubMed: 25557171) »
  • Theivanthiran B  et al. The E3 ubiquitin ligase Itch inhibits p38a signaling and skin inflammation through the ubiquitylation of Tab1. Sci Signal 8:ra22 (2015). Read more (PubMed: 25714464) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MEFs)
Total protein in input
500 µg
Specification
MEFs
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Aug 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up