Recombinant Anti-TAB3 antibody [EPR5965] (ab124723)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5965] to TAB3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TAB3 antibody [EPR5965]
See all TAB3 primary antibodies -
Description
Rabbit monoclonal [EPR5965] to TAB3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide within Human TAB3 aa 50-150. The exact sequence is proprietary.
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Positive control
- HepG2 cells; 293T, HepG2, HeLa, Caco-2, RAW264.7 and NIH 3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5965 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 79 kDa).
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ICC/IF |
1/50.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 79 kDa). |
ICC/IF
1/50. |
Target
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Function
Adapter linking MAP3K7/TAK1 and TRAF6 or TRAF2. Mediator of MAP3K7 activation, respectively in the IL1 and TNF signaling pathways. Plays a role in activation of NF-kappa-B and AP1 transcription factor. Isoform 2 may be an oncogenic factor. -
Tissue specificity
Widely expressed. Constitutively overexpressed in certain tumor tissues. Isoform 1 is a major transcript while isoform 2 is a minor transcript. -
Sequence similarities
Contains 1 CUE domain.
Contains 1 RanBP2-type zinc finger. -
Post-translational
modificationsUbiquitinated; following IL1 stimulation or TRAF6 overexpression. - Information by UniProt
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Database links
- Entrez Gene: 257397 Human
- Entrez Gene: 66724 Mouse
- Omim: 300480 Human
- SwissProt: Q8N5C8 Human
- SwissProt: Q571K4 Mouse
- Unigene: 188256 Human
- Unigene: 119646 Mouse
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Alternative names
- MAP3K7IP 3 antibody
- Mitogen-activated protein kinase kinase kinase 7-interacting protein 3 antibody
- NAP1 antibody
see all
Images
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All lanes : Anti-TAB3 antibody [EPR5965] (ab124723) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 79 kDa -
ab124723, at 1/50 dilution, staining TAB3 in HepG2 cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124723 has been referenced in 3 publications.
- Liu B et al. Downregulation of microRNA-30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients. Mol Med Rep 18:5799-5806 (2018). PubMed: 30365083
- Ren S et al. Hepatitis B Virus Stimulated Fibronectin Facilitates Viral Maintenance and Replication through Two Distinct Mechanisms. PLoS One 11:e0152721 (2016). PubMed: 27023403
- Hu MM et al. TRIM38 inhibits TNFa- and IL-1ß-triggered NF-?B activation by mediating lysosome-dependent degradation of TAB2/3. Proc Natl Acad Sci U S A 111:1509-14 (2014). PubMed: 24434549