Anti-TACD2 antibody (ab64320)

Overview

  • Product nameAnti-TACD2 antibody
    See all TACD2 primary antibodies
  • Description
    Rabbit polyclonal to TACD2
  • SpecificityReacts specifically with human 35 kDa Trop 2 protein
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, WB, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, a wide range of other species
  • Immunogen

    Synthetic peptide derived from C terminal domain of TACD2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 37 kDa.
ICC Use at an assay dependent concentration.

Target

  • FunctionMay function as a growth factor receptor.
  • Tissue specificityPlacenta, pancreatic carcinoma cell lines.
  • Involvement in diseaseDefects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
  • Sequence similaritiesBelongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    • Epithelial glycoprotein 1 antibody
    • GA733 1 antibody
    • GA7331 antibody
    • M1S 1 antibody
    • M1S1 antibody
    • Membrane component chromosome 1 surface marker 1 antibody
    • Pancreatic carcinoma marker protein GA733 1 antibody
    • Pancreatic carcinoma marker protein GA733-1 antibody
    • Pancreatic carcinoma marker protein GA7331 antibody
    • TACD 2 antibody
    • TACD2_HUMAN antibody
    • TACSTD 2 antibody
    • Tacstd2 antibody
    • Trop 2 antibody
    • Trop2 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 2 antibody
    see all

Anti-TACD2 antibody images

  • ICC/IF image of ab64320 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TACD2 antibody (ab64320)

ab64320 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 08 2012

Vielen Dank für Ihre Anrufe.

Wie versprochen habe ich alle unsere Langerin- bzw. TACD2-Antikörper danach geprüft, ob sie schon einmal mit Schwein getestet wurden - was leider bei keinem der Fall ist.

Daher habe ich di...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (prostate)
Specification prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

Submitted Dec 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (prostate)
Specification prostate
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

Submitted Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"