Overview

  • Product name
    TACE Inhibitor Screening Kit
  • Assay type
    Quantitative
  • Assay time
    0h 20m
  • Product overview

    In Abcam’s TACE inhibitor screening Kit, TACE (tumor necrosis factor-a-converting enzyme) hydrolyzes the specific FRET substrate to release the quenched fluorescent group, which can be detected fluorometrically at Ex/Em = 318/449 nm. In the presence of the potent TACE inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor Control GM6001 is included to compare the efficacy of test inhibitors.

  • Notes

    The TACE (tumor necrosis factor-a-converting enzyme), also called ADAM metallopeptidase domain 17 (ADAM17), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. TACE is believed to be involved in the processing of tumor necrosis factor alpha (TNF-a) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-a), and is of known physiological importance.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab155889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

Protocols

References

ab155889 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The TACE enzyme in the ab155889 assay is human recombinant protein, 20ug per vial.

The protocol for the ab155889 TACE Inhibitor Screening Kit has been designed for working with the assay buffer. For it to work with the cell culture media, some individual further optimization may be required.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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