The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000.
1/1000. Predicted molecular weight: 213 kDa.
TAF1 functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.
Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.
Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.
Western blot - Anti-TAF1 antibody (ab188427)
Anti-TAF1 antibody (ab188427) at 1/1000 dilution + HeLa whole cell extract at 10 µg
Predicted band size : 213 kDa
Immunocytochemistry/ Immunofluorescence - Rabbit Polyclonal to TAF1 (ab188427)
IImmunofluorescence staining of TAF1 in in BHK cells (fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40) with ab188427 at 1/1000 dilution. Secondary staining with Goat anti-Rabbit IgG conjugated with rhodamine.
Panel a): Stained with ab188427 without immunizing peptide. Panel b): Stained with ab188427 with immunizing peptide. Panel c): Panel a) co-stained with Hoechst 33258. Panel d): Panel b) co-stained with Hoechst 33258. Panel e): Phase-contrast image of panels a) and c). Panel f): Phase-contrast image of panels b) and d).
Sekiguchi T et al. The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol Cell Biol11:3317-25 (1991).
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