Overview

  • Product name
    Anti-TAF1 antibody
  • Description
    Rabbit polyclonal to TAF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Human TAF1 aa 103-123 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    STEDAVDYSDINEVAEDESRR-C


    Database link: P21675

  • Positive control
    • HeLa whole cell extract; BHK and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab188427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
WB 1/1000. Predicted molecular weight: 213 kDa.

Target

  • Relevance
    TAF1 functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • BA2R antibody
    • CCG 1 antibody
    • CCG1 antibody
    • CCGS antibody
    • Cell cycle G1 phase defect antibody
    • Cell cycle gene 1 protein antibody
    • Complementation of cell cycle block G1 to S antibody
    • DYT3 protein antibody
    • NSCL 2 antibody
    • NSCL2 antibody
    • OF antibody
    • p250 antibody
    • TAF 1 antibody
    • TAF(II)250 antibody
    • TAF1 RNA polymerase II antibody
    • TAF130 antibody
    • TAF145 antibody
    • TAF2A antibody
    • TAFII 250 antibody
    • TAFII250 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
    • TATA box binding protein (TBP)-associated factor antibody
    • TBP associated factor 1 antibody
    • TBP associated factor 145 kDa antibody
    • TBP associated factor 250 kDa antibody
    • Transcription factor TFIID p250 polypeptide antibody
    • Transcription initiation factor TFIID 250 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 1 antibody
    • YGR274C antibody
    see all

Images

  • Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.

  • Anti-TAF1 antibody (ab188427) at 1/1000 dilution + HeLa whole cell extract at 10 µg

    Predicted band size: 213 kDa

  • IImmunofluorescence staining of TAF1 in in BHK cells (fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40) with ab188427 at 1/1000 dilution. Secondary staining with Goat anti-Rabbit IgG conjugated with rhodamine.

    Panel a):  Stained with ab188427 without immunizing peptide.
    Panel b):  Stained with ab188427 with immunizing peptide.
    Panel c):  Panel a) co-stained with Hoechst 33258.
    Panel d):  Panel b) co-stained with Hoechst 33258.
    Panel e):  Phase-contrast image of panels a) and c).
    Panel f):   Phase-contrast image of panels b) and d).

References

This product has been referenced in:
  • Sekiguchi T  et al. The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol Cell Biol 11:3317-25 (1991). WB, ICC/IF . Read more (PubMed: 2038334) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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