Overview

  • Product name
  • Description
    Rabbit polyclonal to TAF11
  • Specificity
    This antibody reacts with TAF11
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Chimpanzee
  • Immunogen

    A region within synthetic peptide: MDDAHESPSD KGGETGESDE TAAVPGDPGA TDTDGIPEET DGDADVDLKE, corresponding to amino acids 1-50 of Human TAF11

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • Function
    Core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
  • Sequence similarities
    Belongs to the TAF11 family.
  • Domain
    TBP and TAFII18 bind to distinct domains of TAFII28.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC:15243 antibody
    • p30 beta antibody
    • PRO2134 antibody
    • TAF antibody
    • TAF(II)28 antibody
    • TAF11 antibody
    • TAF11 RNA polymerase II TATA box binding protein (TBP) associated factor 28 kD antibody
    • TAF11_HUMAN antibody
    • TAF2I antibody
    • TAFII 28 antibody
    • TAFII-28 antibody
    • TAFII28 antibody
    • TFIID subunit p30 beta antibody
    • TFIID subunit p30-beta antibody
    • Transcription initiation factor TFIID 28 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 11 antibody
    see all

Anti-TAF11 antibody images

  • ab50557 staining TAF11 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/150 in TBS + 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1 :
    Lane 2 : Anti-TAF11 antibody (ab50557) at 0.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : Transfected 293T cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)

References for Anti-TAF11 antibody (ab50557)

This product has been referenced in:
  • Anish R  et al. Characterization of transcription from TATA-less promoters: identification of a new core promoter element XCPE2 and analysis of factor requirements. PLoS One 4:e5103 (2009). WB, IP ; Human . Read more (PubMed: 19337366) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample
Dog Tissue sections (MDCK pellets in paraffin)
Specification
MDCK pellets in paraffin
Permeabilization
No
Fixative
Formaldehyde
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Submitted Oct 20 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9
Sample
Human Tissue sections (Infantile fibromatosis)
Specification
Infantile fibromatosis
Permeabilization
No
Fixative
Formaldehyde
Username

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Verified customer

Submitted Jul 18 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
1% FBS/BSA in PBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH9
Sample
Mouse Tissue sections (mouse embryo 18dpc)
Specification
mouse embryo 18dpc
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 25 2014

Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (293T cells)
Specification
293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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