Overview

  • Product nameAnti-TAF15 antibody [4D71]
    See all TAF15 primary antibodies
  • Description
    Mouse monoclonal [4D71] to TAF15
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human TAF15, expressed in and purified from E. coli.

  • Positive control
    • Crude HeLa cell homogenate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionRNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
  • Tissue specificityUbiquitous. Observed in all fetal and adult tissues.
  • Involvement in diseaseNote=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
  • Sequence similaritiesBelongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa TATA-binding protein-associated factor antibody
    • Npl3 antibody
    • RBP56 antibody
    • RBP56_HUMAN antibody
    • RNA binding protein 56 antibody
    • RNA-binding protein 56 antibody
    • TAF antibody
    • TAF(II)68 antibody
    • TAF15 antibody
    • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
    • TAF2N antibody
    • TAFII68 antibody
    • TATA-binding protein-associated factor 2N antibody
    see all

Anti-TAF15 antibody [4D71] images

  • Anti-TAF15 antibody [4D71] (ab124746) at 1 µg/ml + Crude HeLa cell homogenate

    Predicted band size : 62 kDa
  • ab124746, at 1 µg/ml, staining TAF15 (green) in HeLa cell cultures by Immunofluorescence. Blue is a DNA stain and red is Vimentin.

References for Anti-TAF15 antibody [4D71] (ab124746)

ab124746 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab124746.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"