Overview

  • Product name
    Anti-TAF15 antibody [4D71]
    See all TAF15 primary antibodies
  • Description
    Mouse monoclonal [4D71] to TAF15
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human TAF15, expressed in and purified from E. coli.

  • Positive control
    • Crude HeLa cell homogenate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
  • Tissue specificity
    Ubiquitous. Observed in all fetal and adult tissues.
  • Involvement in disease
    Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
  • Sequence similarities
    Belongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa TATA-binding protein-associated factor antibody
    • Npl3 antibody
    • RBP56 antibody
    • RBP56_HUMAN antibody
    • RNA binding protein 56 antibody
    • RNA-binding protein 56 antibody
    • TAF antibody
    • TAF(II)68 antibody
    • TAF15 antibody
    • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
    • TAF2N antibody
    • TAFII68 antibody
    • TATA-binding protein-associated factor 2N antibody
    see all

Images

  • Anti-TAF15 antibody [4D71] (ab124746) at 1 µg/ml + Crude HeLa cell homogenate

    Predicted band size : 62 kDa
  • ab124746, at 1 µg/ml, staining TAF15 (green) in HeLa cell cultures by Immunofluorescence. Blue is a DNA stain and red is Vimentin.

References

ab124746 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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