Overview

  • Product nameAnti-TAF15 antibody
    See all TAF15 primary antibodies
  • Description
    Rabbit polyclonal to TAF15
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from an internal region of human TAF15.

  • Positive control
    • IHC-P: Human brain tissue WB: Extracts from HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab69581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • FunctionRNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
  • Tissue specificityUbiquitous. Observed in all fetal and adult tissues.
  • Involvement in diseaseNote=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
  • Sequence similaritiesBelongs to the RRM TET family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa TATA-binding protein-associated factor antibody
    • Npl3 antibody
    • RBP56 antibody
    • RBP56_HUMAN antibody
    • RNA binding protein 56 antibody
    • RNA-binding protein 56 antibody
    • TAF antibody
    • TAF(II)68 antibody
    • TAF15 antibody
    • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
    • TAF2N antibody
    • TAFII68 antibody
    • TATA-binding protein-associated factor 2N antibody
    see all

Anti-TAF15 antibody images

  • All lanes : Anti-TAF15 antibody (ab69581) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells, without immunising peptide
    Lane 2 : extracts from HepG2 cells, with immunising peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 70 kDa,>117 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of TAF15 in paraffin embedded human brain tissue using 1/50 ab69581. Left image: untreated sample. Right image: sample treated with immunising peptide.

References for Anti-TAF15 antibody (ab69581)

ab69581 has not yet been referenced specifically in any publications.

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