The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
Use at 1-4 µg/mg of lysate.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionRNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Tissue specificityUbiquitous. Observed in all fetal and adult tissues.
Involvement in diseaseNote=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
Sequence similaritiesBelongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
Post-translational modificationsDimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling TAF15 with ab70389 at 1/5000 (0.2µg/ml). Detection: DAB.
Western blot - TAF15 antibody (ab70389)
All lanes : Anti-TAF15 antibody (ab70389) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells
at 200 µg Lane 2 : Whole cell lysate from 293T cells immunoprecipitated with rabbit IgG control. Lane 3 : Whole cell lysate from 293T cells immunoprecipitated with 0.3 µg/ml ab70389.