Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
Flow Cyt: 1/10.
IP: Use at 5µg/0.5mg of lysate.
WB: 1/50. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRegulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
Ab50990 used at 5µg/0.5mg RIPA lysate of HeLa cells.
The immunoprecipitate was subjected to Western blotting using ab50990 at 1/100. The ECL technique was used, and the secondary antibody was Mouse IgG at 1/2500.