Overview

  • Product nameAnti-TAF1C antibody
    See all TAF1C primary antibodies
  • Description
    Mouse monoclonal to TAF1C
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 761-870 of Human TAF1C (NP_005670) with tag.

  • Positive control
    • TAF1C transfected 293T cell lysate. Partial recombinant tagged TAF1C protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab77033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 96 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionComponent of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • RNA polymerase I-specific TBP-associated factor 110 kDa antibody
    • SL1 antibody
    • Taf1c antibody
    • TAF1C_HUMAN antibody
    • TAFI110 antibody
    • TAFI95 antibody
    • TATA box binding protein associated factor 1C antibody
    • TATA box-binding protein-associated factor 1C antibody
    • TATA box-binding protein-associated factor RNA polymerase I subunit C antibody
    • TBP associated factor 1C antibody
    • TBP associated factor RNA polymerase I 95 kDa antibody
    • TBP associated factor, RNA polymerase I, 110-KD antibody
    • TBP-associated factor 1C antibody
    • Transcription initiation factor SL1/TIF-IB subunit C antibody
    see all

Anti-TAF1C antibody images

  • Anti-TAF1C antibody (ab77033) at 1 µg/ml + Partial recombinant tagged TAF1C protein at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 96 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TAF1C antibody (ab77033) at 1 µg/ml

    Lane 1 : TAF1C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 96 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-TAF1C antibody (ab77033)

ab77033 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"