Anti-TAF2 antibody - ChIP Grade (ab86327)

Overview

  • Product nameAnti-TAF2 antibody - ChIP Grade
    See all TAF2 primary antibodies
  • Description
    Rabbit polyclonal to TAF2 - ChIP Grade
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 540 - 589 (KRNVLELEIK QDYTSPGTQK YVGPLKVTVQ ELDGSFNHTL QIEENSLKHD) of Human TAF2 (NP_003175)

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 137 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

Target

  • RelevanceInitiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF2 is one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 150 kDa cofactor of initiator function antibody
    • 150kDa antibody
    • 4732460C16Rik antibody
    • AI425886 antibody
    • CIF150 antibody
    • Cofactor of initiator function, 150kD subunit antibody
    • MGC166014 antibody
    • RNA polymerase II TBP associated factor subunit B 3 antibody
    • RNA polymerase II TBP associated factor subunit B antibody
    • TAF(II)150 antibody
    • TAF2 RNA polymerase II, TATA box binding protein (TBP) associated factor antibody
    • TAF2 RNA polymerase II, TATA box binding protein (TBP) associated factor, 150kDa antibody
    • TAF2 RNA polymerase II, TATA box binding protein-associated factor, 150 KD antibody
    • TAF2B antibody
    • TAFII 150 antibody
    • TAFII150 antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase II, B, 150kD antibody
    • TATA box binding protein associated factor 2B antibody
    • TBP associated factor 150 kDa antibody
    • TBP associated factor, RNA polymerase II, 150 KD antibody
    • TBP-associated factor 2 antibody
    • Transcription initiation factor TFIID 150 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 2 antibody
    see all

Anti-TAF2 antibody - ChIP Grade images

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-TAF2 antibody - ChIP Grade (ab86327) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 137 kDa
    6-18% agarose gel

References for Anti-TAF2 antibody - ChIP Grade (ab86327)

ab86327 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (8% SDS-Page)
Sample Mouse Tissue lysate - whole (Testis)
Specification Testis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Apr 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"