Overview

  • Product name
  • Description
    Rabbit polyclonal to Taf4
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment which was over-expressed in E.coli using a plasmid carrying the N-terminal domain of Taf4 protein (1-200aa) of Saccharomyces cerevisiae and purified by chromatography.

  • Positive control
    • Extract of budding yeast cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • TAF2C antibody
      • TAF2C1 antibody
      • TAF4A antibody
      • TAFII 130 antibody
      • TAFII 135 antibody
      • TBP associated factor 4 antibody
      see all

    Images

    • All lanes : Anti-Taf4 antibody (ab63910) at 1/5000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Extract of budding yeast


      Predicted band size : 42 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 39 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Seizl M  et al. Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization. Nucleic Acids Res : (2011). WB ; Saccharomyces cerevisiae . Read more (PubMed: 21498544) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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