Overview

  • Product nameAnti-TAF6 antibody [585D4a]
    See all TAF6 primary antibodies
  • Description
    Mouse monoclonal [585D4a] to TAF6
  • Tested applicationsSuitable for: WB, IP, ICC, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment

  • Positive control
    • HEK293 whole cell lysate. HeLa cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified using protein G chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • ClonalityMonoclonal
  • Clone number585D4a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IP Use at 10 µg/mg of lysate.
ICC 1/5.
Dot blot Use at an assay dependent dilution.

Target

  • RelevanceTAF6 is a subunit of transcription factor IID (TFIID). Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • TAF2E antibody
    • E 70/85kD antibody
    • TAF 6 antibody
    • TAF6 RNA polymerase II TATA box binding protein (TBP) associated factor 80 kD antibody
    • TAF6 RNA polymerase II, TATA box binding protein associated factor, 80kDa antibody
    • TAFII 70 antibody
    • TAFII80 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II E 70/85kD antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase II, antibody
    • TBP associated factor 6 antibody
    • Transcription initiation factor TFIID 70 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 6 antibody
    see all

Anti-TAF6 antibody [585D4a] images

  • Anti-TAF6 antibody [585D4a] (ab51026) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.
  • ab51026 was used at 5µg/500µg of RIPA lysate of HeLa cells. Western blot was labelled with ab51026 at 1/50 dilution followed by Mouse IgG antibody at 1/2500 dilution and developed using the ECL technique.
  • HeLa cells labelled with ab51026 at 1/5 dilution. Alexa Fluor 488 Goat anti-Mouse IgG was used as secondary antibody at 1/200 dilution.

References for Anti-TAF6 antibody [585D4a] (ab51026)

This product has been referenced in:
  • Sela D  et al. Endoplasmic Reticulum Stress-Responsive Transcription Factor ATF6a Directs Recruitment of the Mediator of RNA Polymerase II Transcription and Multiple Histone Acetyltransferase Complexes. J Biol Chem : (2012). WB . Read more (PubMed: 22577136) »
  • Takahashi H  et al. Proteomics Reveals a Physical and Functional Link between Hepatocyte Nuclear Factor 4{alpha} and Transcription Factor IID. J Biol Chem 284:32405-12 (2009). WB ; Human . Read more (PubMed: 19805548) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"