Overview

  • Product name
  • Description
    Rabbit polyclonal to TAF6
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant protein, corresponding to N terminal amino acids 1-200 of TAF6 of budding yeast.

  • Positive control
    • Extract of budding yeast

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
  • Purity
    Whole antiserum
  • Purification notes
    ab64060 was purified by chromatography
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 72 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    TAF6 is a subunit of transcription factor IID (TFIID). Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
  • Cellular localization
    Nuclear
  • Alternative names
    • TAF2E antibody
    • E 70/85kD antibody
    • TAF 6 antibody
    • TAF6 RNA polymerase II TATA box binding protein (TBP) associated factor 80 kD antibody
    • TAF6 RNA polymerase II, TATA box binding protein associated factor, 80kDa antibody
    • TAFII 70 antibody
    • TAFII80 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II E 70/85kD antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase II, antibody
    • TBP associated factor 6 antibody
    • Transcription initiation factor TFIID 70 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 6 antibody
    see all

Images

References

ab64060 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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