The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 72 kDa).
Use at an assay dependent dilution.
RelevanceTAF6 is a subunit of transcription factor IID (TFIID).
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.
TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
E 70/85kD antibody
TAF 6 antibody
TAF6 RNA polymerase II TATA box binding protein (TBP) associated factor 80 kD antibody
TAF6 RNA polymerase II, TATA box binding protein associated factor,
TAFII 70 antibody
TATA box binding protein (TBP) associated factor RNA polymerase II E 70/85kD antibody
TATA box binding protein (TBP) associated factor, RNA polymerase II, antibody