Overview

  • Product nameAnti-TAF6 antibody
    See all TAF6 primary antibodies
  • Description
    Rabbit polyclonal to TAF6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    A region within synthetic peptide: LLTDEVSYRI KEIAQDALKF MHMGKRQKLT TSDIDYALKL KNVEPLYGFH, corresponding to N terminal amino acids 38-87 of Human TAF6

  • Positive control
    • Human Kidney tissue, transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab64423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceTAF6 is a subunit of transcription factor IID (TFIID). Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • TAF2E antibody
    • E 70/85kD antibody
    • TAF 6 antibody
    • TAF6 RNA polymerase II TATA box binding protein (TBP) associated factor 80 kD antibody
    • TAF6 RNA polymerase II, TATA box binding protein associated factor, 80kDa antibody
    • TAFII 70 antibody
    • TAFII80 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II E 70/85kD antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase II, antibody
    • TBP associated factor 6 antibody
    • Transcription initiation factor TFIID 70 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 6 antibody
    see all

Anti-TAF6 antibody images

  • Anti-TAF6 antibody (ab64423) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TAF6 with ab64423 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

References for Anti-TAF6 antibody (ab64423)

This product has been referenced in:
  • D'Alessio JA  et al. Core promoter recognition complex changes accompany liver development. Proc Natl Acad Sci U S A 108:3906-11 (2011). WB ; Mouse . Read more (PubMed: 21368148) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"