• Product nameAnti-TAF6 antibody
    See all TAF6 primary antibodies
  • Description
    Rabbit polyclonal to TAF6
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 627 and 677 of human TAF6 (NP_005632.1)

  • Positive control
    • HeLa and 293T whole cell lysates.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab76922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • RelevanceTAF6 is a subunit of transcription factor IID (TFIID). Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • TAF2E antibody
    • E 70/85kD antibody
    • TAF 6 antibody
    • TAF6 RNA polymerase II TATA box binding protein (TBP) associated factor 80 kD antibody
    • TAF6 RNA polymerase II, TATA box binding protein associated factor, 80kDa antibody
    • TAFII 70 antibody
    • TAFII80 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II E 70/85kD antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase II, antibody
    • TBP associated factor 6 antibody
    • Transcription initiation factor TFIID 70 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 6 antibody
    see all

Anti-TAF6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TAF6 with ab76922 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TAF6 antibody (ab76922) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size : 73 kDa
    Observed band size : 94 kDa (why is the actual band size different from the predicted?)

    Exposure time : 10 seconds
  • Detection of TAF6 by Western Blot of Immunprecipitate.
    ab76922 at 1µg/ml staining TAF6 in HeLa whole cell lysate immunoprecipitated using ab76922 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-TAF6 antibody (ab76922)

ab76922 has not yet been referenced specifically in any publications.

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