Overview

  • Product name
    Anti-TAF7 antibody
  • Description
    Mouse monoclonal to TAF7
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: FIWNHGITLP LKNVRKRRFR KTAKKKYIES PDVEKEVKRL LSTDAEAVST RWEIIAEDET KEAENQGLDI SSPGMSGHRQ GHDSLEHDEL REIFN, corresponding to amino acids 130-224 of Human TAF7

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab57494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the TAF7 family.
  • Domain
    The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • RNA polymerase II TBP-associated factor subunit F antibody
    • TAF 7 antibody
    • TAF(II)55 antibody
    • TAF2F antibody
    • TAF7 antibody
    • TAF7 RNA polymerase II antibody
    • TAF7 RNA polymerase II TATA box binding protein (TBP) associated factor 55 kDa antibody
    • TAF7_HUMAN antibody
    • TAFII 55 antibody
    • TAFII-55 antibody
    • TAFII55 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II F 55kD antibody
    • TATA box binding protein associated factor 2F antibody
    • TBP associated factor F antibody
    • Transcription factor IID subunit TAFII55 antibody
    • Transcription initiation factor TFIID 55 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 7 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab57494 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57494, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • TAF7 antibody (ab57494) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymph node.


  • Predicted band size : 40 kDa
    TAF7 antibody (ab57494) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • Lane 2 : Anti-TAF7 antibody (ab57494) at 1/1250 dilution
    Lane 3 : Anti-TAF7 antibody (ab57494) at 1/2500 dilution
    Lane 4 : Anti-TAF7 antibody (ab57494) at 1/5000 dilution
    Lane 5 : Anti-TAF7 antibody (ab57494) at 1/10000 dilution

    Lane 2 : whole cell lysate prepared from SW780 badder cancer cells
    Lane 3 : whole cell lysate prepared from SW780 badder cancer cells
    Lane 4 : whole cell lysate prepared from SW780 badder cancer cells
    Lane 5 : whole cell lysate prepared from SW780 badder cancer cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG conjugated to HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview

    Lane 1: All Blue Biorad.
    Gel run under denaturing conditions with a 4-12% gradient.
    Blocking step performed using 5% milk for one hour at room temperature.
    Primary antibody incubated for 16 hours.

    See Abreview

  • ab57494 staining TAF7 in human bladder cancer tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TEG. Samples were then blocked with 1% serum for 1 hour at room temperature followed by incubation with the primary antibody at a 1/3500 dilution for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Gegonne A  et al. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Mol Cell Biol 32:1984-97 (2012). WB . Read more (PubMed: 22411629) »
  • Devaiah BN & Singer DS Cross-talk Among RNA Polymerase II Kinases Modulates C-terminal Domain Phosphorylation. J Biol Chem 287:38755-66 (2012). WB . Read more (PubMed: 23027873) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bladder cancer)
Specification
bladder cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TEG
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

Submitted May 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SW780 bladder cancer)
Loading amount
25 µg
Specification
SW780 bladder cancer
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

Submitted Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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