Overview

  • Product nameAnti-TAF7L antibody
    See all TAF7L primary antibodies
  • Description
    Rabbit polyclonal to TAF7L
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse TAF7L aa 357-406 (internal sequence).
    Sequence:

    DEDY GNEKEEEETD NSEEELEKEL QAKFNEFSLH EADQDYSSIT MAIQKL

  • Positive control
    • SP2/0 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

Target

  • FunctionProbably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis.
  • Tissue specificityTestis-specific.
  • Sequence similaritiesBelongs to the TAF7 family.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic in spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into the nuclei of pachytene spermatocytes and round spermatids.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 40 antibody
    • CT40 antibody
    • FLJ23157 antibody
    • RNA polymerase II TBP-associated factor subunit Q antibody
    • TAF2Q antibody
    • TAF7 like RNA polymerase II, TATA box binding protein (TBP) associated factor antibody
    • Taf7l antibody
    • TAF7L_HUMAN antibody
    • TATA box binding protein-associated factor, RNA polymerase II, Q antibody
    • TATA box-binding protein-associated factor 50 kDa antibody
    • TBP associated factor, RNA polymerase II, Q antibody
    • Transcription initiation factor TFIID 50 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 7-like antibody
    see all

Anti-TAF7L antibody images

  • Anti-TAF7L antibody (ab49164) at 1.25 µg/ml + SP2\0 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References for Anti-TAF7L antibody (ab49164)

This product has been referenced in:
  • D'Alessio JA  et al. Core promoter recognition complex changes accompany liver development. Proc Natl Acad Sci U S A 108:3906-11 (2011). WB ; Mouse . Read more (PubMed: 21368148) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab49164.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"