Overview

  • Product name
  • Description
    Rabbit polyclonal to TAF7L
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse TAF7L aa 357-406 (internal sequence).
    Sequence:

    DEDY GNEKEEEETD NSEEELEKEL QAKFNEFSLH EADQDYSSIT MAIQKL

  • Positive control
    • SP2/0 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

Target

  • Function
    Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis.
  • Tissue specificity
    Testis-specific.
  • Sequence similarities
    Belongs to the TAF7 family.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasmic in spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into the nuclei of pachytene spermatocytes and round spermatids.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 40 antibody
    • CT40 antibody
    • FLJ23157 antibody
    • RNA polymerase II TBP-associated factor subunit Q antibody
    • TAF2Q antibody
    • TAF7 like RNA polymerase II, TATA box binding protein (TBP) associated factor antibody
    • Taf7l antibody
    • TAF7L_HUMAN antibody
    • TATA box binding protein-associated factor, RNA polymerase II, Q antibody
    • TATA box-binding protein-associated factor 50 kDa antibody
    • TBP associated factor, RNA polymerase II, Q antibody
    • Transcription initiation factor TFIID 50 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 7-like antibody
    see all

Images

  • Anti-TAF7L antibody (ab49164) at 1.25 µg/ml + SP2\0 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References

This product has been referenced in:
  • D'Alessio JA  et al. Core promoter recognition complex changes accompany liver development. Proc Natl Acad Sci U S A 108:3906-11 (2011). WB ; Mouse . Read more (PubMed: 21368148) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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