• Product name
  • Description
    Rabbit polyclonal to TAF8
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 260-310 of Human TAF8 (NP_612639.2).

  • Positive control
    • 293T whole cell lysate.



Our Abpromise guarantee covers the use of ab114114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.
    • Sequence similarities
      Belongs to the TAF8 family.
      Contains 1 histone-fold domain.
    • Domain
      Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.
    • Cellular localization
      Nucleus. Cytoplasm. Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ32821 antibody
      • hTAFII43 antibody
      • Protein taube nuss antibody
      • TAF(II)43 antibody
      • taf8 antibody
      • TAF8 RNA polymerase II, TATA box binding protein (TBP) associated factor, 43kDa antibody
      • TAF8_HUMAN antibody
      • TAFII-43 antibody
      • TAFII43 antibody
      • TATA box binding protein (TBP) associated factor, RNA polymerase II A 45/50kDa antibody
      • Taube nuss homolog (mouse) antibody
      • TBN antibody
      • TBP associated factor 8 antibody
      • TBP associated factor TAFII43 antibody
      • TBP associated factor, RNA polymerase II, 43 kD antibody
      • TBP-associated factor 43 kDa antibody
      • TBP-associated factor 8 antibody
      • Transcription initiation factor TFIID 43 kDa subunit antibody
      • Transcription initiation factor TFIID subunit 8 antibody
      see all


    • Detection of Human TAF8 by Western blot of Immunoprecipitate.
      TAF8 was immunoprecipitated from 293T whole cell lysate, using ab114114 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). ab114114 was used for the subsequent WB at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 30 seconds.


    ab114114 has not yet been referenced specifically in any publications.

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