Overview

  • Product nameAnti-TAF8 antibody
    See all TAF8 primary antibodies
  • Description
    Mouse polyclonal to TAF8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human TAF8 protein (AAH33728.1, or Q7Z7C8-3)

  • Positive control
    • TAF8 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 19 kDa for isoform 3 and 34 kDa for isoform 1.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.
    • Sequence similaritiesBelongs to the TAF8 family.
      Contains 1 histone-fold domain.
    • DomainEctopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.
    • Cellular localizationNucleus. Cytoplasm. Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ32821 antibody
      • hTAFII43 antibody
      • Protein taube nuss antibody
      • TAF(II)43 antibody
      • taf8 antibody
      • TAF8 RNA polymerase II, TATA box binding protein (TBP) associated factor, 43kDa antibody
      • TAF8_HUMAN antibody
      • TAFII-43 antibody
      • TAFII43 antibody
      • TATA box binding protein (TBP) associated factor, RNA polymerase II A 45/50kDa antibody
      • Taube nuss homolog (mouse) antibody
      • TBN antibody
      • TBP associated factor 8 antibody
      • TBP associated factor TAFII43 antibody
      • TBP associated factor, RNA polymerase II, 43 kD antibody
      • TBP-associated factor 43 kDa antibody
      • TBP-associated factor 8 antibody
      • Transcription initiation factor TFIID 43 kDa subunit antibody
      • Transcription initiation factor TFIID subunit 8 antibody
      see all

    Anti-TAF8 antibody images

    • All lanes : Anti-TAF8 antibody (ab77059) at 5 µg/ml

      Lane 1 : TAF8 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 19 kDa
      Observed band size : 19 kDa

    References for Anti-TAF8 antibody (ab77059)

    ab77059 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK 293T)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 50 µg
    Specification HEK 293T
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Verified customer

    Submitted Sep 22 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample Human Tissue sections (Human fibromatosis)
    Specification Human fibromatosis
    Permeabilization Yes - Tween-20
    Fixative Formaldehyde
    Username

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    Verified customer

    Submitted Mar 13 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 1°C
    Sample Mouse Cell (MSC)
    Specification MSC
    Permeabilization Yes - Triton 1%
    Fixative Formaldehyde
    Username

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    Verified customer

    Submitted Mar 04 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"