The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/32000 - antibody limit dilution.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Some isoforms may be involved in cardiolipin (CL) metabolism.
High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes.
Involvement in disease
Belongs to the taffazin family.
The hydrophilic domain may serve as an exposed loop interacting with other proteins.