Overview

  • Product nameAnti-TAG72 antibody [CC49]See all TAG72 primary antibodies ...
  • Description
    Mouse monoclonal [CC49] to TAG72
  • SpecificityThis antibody has been shown to be distinct from B72.3 (ab691, ab1320-prediluted) by reciprocal competition radio-immunoassays and it has a Ka higher than that of B72.3.
  • Tested applicationsIHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified).

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab16838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/1.

Target

  • RelevanceThe majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary, and non-small cell lung cancer display some cell populations that are positive for TAG72. About 60% of carcinoma patients express TAG72 in their sera. TAG72 is reportedly useful in distinguishing pulmonary adenocarcinomas from pleural mesotheliomas.
  • Cellular localizationCytoplasmic
  • Alternative names
    • CA 72.4 antibody
    • TAG 72 antibody
    • Tumor associated Glycoprotein 72 antibody

Anti-TAG72 antibody [CC49] images

  • ab16838 staining human colon carcinoma by IHC-P.
  • ICC/IF image of ab16838 stained MCF-7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16838, undiluted) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TAG72 antibody [CC49] (ab16838)

This product has been referenced in:
  • Constantinou P  et al. Extending immunofluorescence detection limits in whole paraffin-embedded formalin fixed tissues using hyperspectral confocal fluorescence imaging. J Microsc 234:137-46 (2009). IHC-P ; Human . Read more (PubMed: 19397743) »
  • Constantinou P  et al. Extending immunofluorescence detection limits in whole paraffin-embedded formalin fixed tissues using hyperspectral confocal fluorescence imaging. J Microsc 234:137-46 (2009). IHC-P ; Human . Read more (PubMed: 19397743) »

See all 2 Publications for this product

Product Wall


TAG72 antibody, ab16838, Lot GR99749-1: 66.6 ug/ml


Epcam antibody (Ber-EP4), ab7504, Lot GR103717-1: 47.3 ug/ml

Thank you for contacting Abcam.

I found some information regarding the conjugation for antibodies that contain BSA through the link below:
http://www.abcam.com/index.html?pageconfig=resource&rid=13156

Our tests have found that...

Read More

Thank you for contacting us.

ab1320 and ab16838 are tissue culture supernatant, not purified antibodies. The concentration to the specific antibodies cannot be determined for these two products.

I hope this information is helpful ...

Read More

Thank you for your reply. Both of these antibodies are tissue culture supernatant meaning that it is not possible to determine antibody concentration, since tissue culture supernatant contains proteins besides the antibody of interest. Supernat...

Read More

Thank you for your enquiry. There's no concentration information available for this antibody as it is provided in an unpurified form (tissue culture supernatant) and we cannot determine the concentration for unpurified products. To use the antibody in ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"