Overview

  • Product name
    Anti-TAGAP antibody [EPR15593]
  • Description
    Rabbit monoclonal [EPR15593] to TAGAP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TAGAP aa 250-400. The exact sequence is proprietary.
    Database link: Q8N103

  • Positive control
    • A549, Jurkat and HepG2 cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/20 - 1/40.
WB 1/1000 - 1/2000. Detects a band of approximately 61, 81 kDa (predicted molecular weight: 81 kDa).
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • 2610315E15Rik antibody
    • ARHGAP47 antibody
    • FKSG15 antibody
    • FLJ32631 antibody
    • FLJ39771 antibody
    • IDDM21 antibody
    • MGC133247 antibody
    • MGC27381 antibody
    • MGC31468 antibody
    • T cell activation GTPase activating protein antibody
    • T cell activation Rho GTPase activating protein antibody
    • T-cell activation GTPase-activating protein antibody
    • T-cell activation Rho GTPase-activating protein antibody
    • Tagap antibody
    • TAGAP_HUMAN antibody
    • TAGAP1 antibody
    see all

Images

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TAGAP with ab187664 at 1/160 dilution (pink) compared to a Rabbit monoclonal IgG Isotype control (green). Goat anti rabbit IgG (FITC) was used as the secondary antibody at 1/150 dilution.

  • All lanes : Anti-TAGAP antibody [EPR15593] (ab187664) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 81 kDa
    Observed band size: 61,81 kDa (why is the actual band size different from the predicted?)



    Based on sequence analysis, ab187664 recognizes three isoforms with the predicted MWs of 81kDa, 61kDa and 57kDa, respectively.

  • Western blot analysis of TAGAP in A549 cell lysate immunoprecipitated with ab187664 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

This product has been referenced in:
  • Berge T  et al. The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by vitamin D in CD4+ T cells. Genes Immun 17:118-27 (2016). Flow Cyt ; Human . Read more (PubMed: 26765264) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187664.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up