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Rabbit polyclonal to TAGLN2
Mouse, Rat, Human
antigen, corresponding to the N-terminal region amino acids 4-136 of the Human TAGLN2 protein (P37802).
Human bone marrow tissue and Human RT-4, U-251 MG, Tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Belongs to the calponin family.
Contains 1 calponin-like repeat. Contains 1 CH (calponin-homology) domain.
Information by UniProt
epididymis tissue protein Li 7e antibody
Immunocytochemistry/ Immunofluorescence - Anti-TAGLN2 antibody (ab121146)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (actin filaments). Recommended concentration of ab121146 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN2 antibody (ab121146)
Immunohistochemical staining of Human bone marrow with ab121146 at 1/500 dilution.
Western blot - Anti-TAGLN2 antibody (ab121146)
All lanes : Anti-TAGLN2 antibody (ab121146) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma cell lysate Lane 4 : Liver cell lysate Lane 5 : Tonsil cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"