• Product nameAnti-TAK1 antibody [EPR5984]
    See all TAK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5984] to TAK1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. within Human TAK1 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O43318

  • Positive control
    • K562, HeLa or A431 cell lysate; Human brain tissue ICC/IF: HAP1 cells and HAP1-TAK1 KO cells
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/1000.
Flow Cyt Use at an assay dependent concentration.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionComponent of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
      Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
    • Information by UniProt
    • Database links
    • Alternative names
      • M3K7_HUMAN antibody
      • MAP3K 7 antibody
      • Map3k7 antibody
      • MEKK7 antibody
      • Mitogen activated protein kinase kinase kinase 7 antibody
      • Mitogen-activated protein kinase kinase kinase 7 antibody
      • TAK1 antibody
      • TGF beta activated kinase 1 antibody
      • TGF-beta-activated kinase 1 antibody
      • TGF1a antibody
      • Transforming growth factor beta activated kinase 1 antibody
      • Transforming growth factor-beta-activated kinase 1 antibody
      see all

    Anti-TAK1 antibody [EPR5984] images

    • Predicted band size : 67 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TAK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: SHSY5Y cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109526 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109526 was shown to specifically react with TAK1 when TAK1 knockout samples were used. Wild-type and TAK1 knockout samples were subjected to SDS-PAGE. ab109526 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • ab109526 staining TAK1 in wild-type HAP1 cells (top panel) and TAK1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab109526 at 1/1000 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling TAK1 with unpurified ab109526 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • All lanes : Anti-TAK1 antibody [EPR5984] (ab109526) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 67 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab109526, at a 1/50 dilution, staining TAK1 in Formalin/PFA-fixed paraffin-embedded Human brain tissue, by Immunohistochemistry.

    References for Anti-TAK1 antibody [EPR5984] (ab109526)

    This product has been referenced in:
    • Cheng J  et al. Inhibition of transforming growth factor ß-activated kinase 1 prevents inflammation-related cartilage degradation in osteoarthritis. Sci Rep 6:34497 (2016). IHC-P . Read more (PubMed: 27682596) »
    • Gu M  et al. Phosphatase holoenzyme PP1/GADD34 negatively regulates TLR response by inhibiting TAK1 serine 412 phosphorylation. J Immunol 192:2846-56 (2014). Read more (PubMed: 24534530) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (hepatocytes)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification hepatocytes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Dec 10 2015

    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (Hep3B)
    Specification Hep3B
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Nov 17 2014