Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

Overview

  • Product nameAnti-TAK1 (phospho S439) antibody [EPR2863]
    See all TAK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2863] to TAK1 (phospho S439)
  • Specificityab109404 only detects TAK1 phosphorylated at Serine 439 in Human or TAK1 phosphorylated at Serine 412 in Mouse.
  • Tested applicationsSuitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho specific peptide against residues surrounding Serine 439 of Human TAK1.

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionComponent of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
      Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
    • Information by UniProt
    • Database links
    • Alternative names
      • M3K7_HUMAN antibody
      • MAP3K 7 antibody
      • Map3k7 antibody
      • MEKK7 antibody
      • Mitogen activated protein kinase kinase kinase 7 antibody
      • Mitogen-activated protein kinase kinase kinase 7 antibody
      • TAK1 antibody
      • TGF beta activated kinase 1 antibody
      • TGF-beta-activated kinase 1 antibody
      • TGF1a antibody
      • Transforming growth factor beta activated kinase 1 antibody
      • Transforming growth factor-beta-activated kinase 1 antibody
      see all

    Anti-TAK1 (phospho S439) antibody [EPR2863] images

    • All lanes : Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with transforming growth factor-beta (TGF-beta)

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 67 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Dot Blot analysis of Lane 1: TAK1 (phospho S439) phospho peptide and Lane 2: TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 3 minutes.

    References for Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

    ab109404 has not yet been referenced specifically in any publications.

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    Unfortunately, I can say that the anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) has been tried in IHC of paraffin embedded tissue sections, however it was found not to be...

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