Anti-Tal1 antibody - C-terminal (ab155195)

Overview

  • Product name
    Anti-Tal1 antibody - C-terminal
    See all Tal1 primary antibodies
  • Description
    Rabbit polyclonal to Tal1 - C-terminal
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 252-331 of Human Tal1 (Uniprot ID: P17542).

  • Positive control
    • 293T, H1299, HeLa, HepG2, MOLT4, Raji whole cell lysate; A549 xenograft
  • General notes
    Keep as a concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.
  • Tissue specificity
    Leukemic stem cell.
  • Involvement in disease
    Note=A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain
    The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.
  • Post-translational
    modifications
    Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.
    Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHa17 antibody
    • Class A basic helix-loop-helix protein 17 antibody
    • OTTHUMP00000009563 antibody
    • OTTHUMP00000009564 antibody
    • SCL antibody
    • STEM CELL LEUKEMIA HEMATOPOIETIC TRANSCRIPTION FACTOR antibody
    • Stem cell protein antibody
    • T cell acute lymphocytic leukemia 1 antibody
    • T cell acute lymphocytic leukemia 1 protein antibody
    • T cell acute lymphocytic leukemia 1 protein antibody
    • T cell leukemia/lymphoma 5 protein antibody
    • T-cell acute lymphocytic leukemia protein 1 antibody
    • T-cell leukemia/lymphoma protein 5 antibody
    • Tal 1 antibody
    • Tal 1 product antibody
    • TAL 1 protein antibody
    • TAL bHLH transcription factor 1 erythroid differentiation factor antibody
    • TAL-1 antibody
    • tal1 antibody
    • TAL1_HUMAN antibody
    • TCL 5 antibody
    • TCL5 antibody
    see all

Images

  • Anti-Tal1 antibody - C-terminal (ab155195) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 34 kDa
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling Tal1 with ab155195 at 1/100 dilution.

References

ab155195 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ChIP
Sample
Human Cell lysate - nuclear (KG1 leukemic cell line)
Negative control
Negative control region near the AFP gene. IP using IgG
Specification
KG1 leukemic cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde in PBS, room t
Positive control
3 positive control regions were used - the ERG +85 enhancer (SCL/TAL1 binding to this region in KG1 cells was published in Diffner et al Blood 2013 PMID:23327922) - plus two additional unpublished regions. IP was done in parallel with the sc12984X antibody used in Diffner et al.
Username

Dr. Julie Thoms

Verified customer

Submitted Feb 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8, 100C, 20min
Sample
Human Tissue sections (intestine)
Specification
intestine
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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